Journal: Scientific Reports

Article Title: Promysalin is a salicylate-containing antimicrobial with a cell-membrane-disrupting mechanism of action on Gram-positive bacteria

doi: 10.1038/s41598-017-07567-0

Figure Lengend Snippet: Membrane damage and cFSE fluorescence leakage in S. thermophilus DSM 20617 T exposed to promysalin, chlorhexidine, and gramicidin. Flow cytometry density diagrams show the cFSE vs PI fluorescence of cells exposed to promysalin or chlorhexidine or gramicidin (100, 200 µg/ml, and 100 µM respectively). ( a ) Cells before cFDASE labelling. ( b ) Cell labelled with cFSE and PI. ( c ) Cells after 60 min exposure to promysalin. Viable cells are gated in G1, viable cells with slightly damaged cell membrane are gated in G2. Dead cells with damaged membrane are gated in G3. The transition of cell population from gate G1 to gate G3 is related to the entity of cell membrane damage. ( d ) Leakage of cFSE fluorescence outside the cells during the exposure to promysalin, chlorhexidine and the membrane uncoupling gramicidin.

Article Snippet: The cFSE fluorescence intensity of stained cells was recovered by flow cytometry in the FL1 channel (excitation 488 nm, emission filter 530/30, provided by BD Biosciences, Milan, Italy).

Techniques: Fluorescence, Flow Cytometry

Journal: Redox Biology

Article Title: Glutaredoxin deficiency promotes activation of the transforming growth factor beta pathway in airway epithelial cells, in association with fibrotic airway remodeling

doi: 10.1016/j.redox.2020.101720

Figure Lengend Snippet: Assessment of inflammation, mucus metaplasia and smooth muscle cells in WT or Glrx -/- mice following repeated exposure to house dust mite. A: Western Blot analysis for GLRX in homogenized lung tissue of WT or Glrx -/- mice exposed to saline or house dust mite (HDM). ACTB (β-actin): loading control. B: Total (left) and differential cell counts (right) in BAL fluid from WT or Glrx -/- mice after exposure to saline or HDM. C: Periodic acid-Schiff (PAS) staining in WT or Glrx -/- mice exposed to HDM or saline (scale bar = 50 μm). Quantification of airway mucus staining intensity was determined by positive staining areas using Metamorph. D: Alpha-smooth muscle actin (ACTA2) immune-reactivity in WT or Glrx -/- mice exposed to saline or HDM (scale bar = 50 μm). WT PBS n = 8, WT HDM n = 10, Glrx -/- PBS n = 8, Glrx -/- HDM n = 10 mice. ***p < 0.001, ANOVA.

Article Snippet: Quantification of airway mucus staining intensity was determined by positive staining areas using Metamorph.

Techniques: Western Blot, Saline, Control, Staining